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KMID : 0624620140470100581
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BMB Reports
2014 Volume.47 No. 10 p.581 ~ p.586
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YAC tripeptide of epidermal growth factor promotes the proliferation of HaCaT keratinocytes through activation of EGFR
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Yoo Yeon-Ho
Kim Yu-Ri
Kim Min-Seo
Lee Kyoung-Jin
Park Kyeong-Han
Hahn Jang-Hee
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Abstract
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Epidermal growth factor (EGF) is known to play key roles in skin regeneration and wound-healing. Here, we demonstrate that Pep2-YAC, a tripeptide covering residues 29-31 in the B loop of EGF, promotes the proliferation of HaCaT keratinocytes with activity comparable to EGF. The treatment of HaCaT cells with Pep2-YAC induced phosphorylation, internalization, and degradation of EGFR and organization of signaling complexes, which consist of Grb2, Gab1, SHP2, and PI3K. In addition, it stimulated the phosphorylation of ERK1/2 at Thr 202/Tyr 204 and of Akt1 at Ser 473 and the nuclear translocation of EGFR, STAT3, c-Jun, and c-Fos. These results suggest that Pep2-YAC may be useful as a therapeutic agent for skin regeneration and wound-healing as an EGFR agonist.
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KEYWORD
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EGF, EGFR, Keratinocyte proliferation, Skin regeneration, Tripeptide
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